What appears to be happening here is that when the user invokes the Screenshot Tools Audacity then (without warning and without asking the user) resets the Toolbar positions and visible set of Toolbars to default - thereby removing the user's carefully set up Toolbar selection and positioning.

It does not appear to be a full reset as it does not seem to change the settings of values in the Toolbars (e.g. Selection Toolbar, Device Toolbar etc.)

a) I believe it is both wrong and probably unnecessary for Screenshot Tools to meddle with the users Toolbars - and as such I believe this warrants P3 rating and a Release Note.

b) Without the enhancement Proposal that I have been working on for Custom resets for Toolbar layouts there is no easy way for the user to simply restore their preferred layout:  See:
http://wiki.audacityteam.org/wiki/Proposal:_GUI_Custom_Resets_for_Toolbar_Layouts

c) The user may well be trying to make a screenshot showing a particular Toolbar layout - Screenshot Tools makes that impossible.

DEVEL - FIX MADE
https://github.com/audacity/audacity/commit/88cafa7a8971ad7f10aed693b7051bfd55ae0378

I also checked that Bug 382 hadn't come back with this change.

(In reply to James Crook from comment #2)
> DEVEL - FIX MADE https://github.com/audacity/audacity/commit/88cafa7a8971ad7f10aed693b7051bfd55ae0378
> I also checked that Bug 382 hadn't come back with this change.

In my testing on Windows 10, the two "Resize..." buttons near the top of Screenshot Tools still reset toolbars to defaults on opening a new project or restarting Audacity, though this is not apparent in the checkboxes in View > Toolbars until the new project window is opened. Can we fix that too?

OK.  Re Comment #3, we now no longer reset toolbars in that case either.
https://github.com/audacity/audacity/commit/6cea9822c3064cb83574e7a137c75ffa166d1b4c

"Residual" fixed.